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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in many tumors, which leads to chromosome instability, and therefore could contribute to tumorigenesis. This protein was uncovered to affiliate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a vital purpose in selling cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have already been explained. Two added splice variants had been described but detailed nucleotide sequence details is not but obtainable.
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Blocking peptides are peptides that bind precisely into the target antibody and block antibody binding. These peptide normally contains the epitope acknowledged through the antibody.
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This guidebook outlines further more possible optimization of mobile and immunoassay parameters to guarantee the absolute best outcomes are...
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This guidebook outlines even more doable optimization of mobile and immunoassay parameters to guarantee the very best effects are...
Antibodies sure to the blocking peptide now not bind to your epitope to the focus on protein. By evaluating the staining through the blocked antibody compared to the antibody by itself, you can see which staining is unique.
Goal Information Cyclin E belongs to your extremely conserved cyclin relatives, whose users are characterised by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute into the temporal coordination of every mitotic function. Cyclin E forms a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S section boundary which is degraded as cells progress through S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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